Journal: Journal of Ginseng Research

Article Title: Stereospecific anticancer effects of ginsenoside Rg3 epimers isolated from heat-processed American ginseng on human gastric cancer cell

doi: 10.1016/j.jgr.2013.11.007

Figure Lengend Snippet: Changes in effects of American ginseng upon heat-processing on AGS cell proliferation. (A) Morphological changes were confirmed using phase-contrast microscopy. (B) Cells were treated with American ginseng with or without heat-processing at different concentrations (150 μg/mL, 300 μg/mL, and 600 μg/mL) for 24 h. Relative cell proliferation was measured by the CCK-8 assay. Each value represents the mean ± standard deviation of three independent experiments. (C) Schematic description for the preparation of methanol-soluble fraction with HP 20 column chromatography. (D) Cells were treated with water eluate or methanol eluate at different concentrations (12.5 μg/mL, 25 μg/mL, 50 μg/mL, and 100 μg/mL) for 24 h. Relative cell proliferation was measured by the CCK-8 assay. Each value represents the mean ± standard deviation of three independent experiments. * p < 0.05 compared with the vehicle control. CCK-8, Cell Counting Kit-8.

Article Snippet: The human gastric cancer AGS cell line was purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Microscopy, CCK-8 Assay, Standard Deviation, Column Chromatography, Control, Cell Counting

Journal: Journal of Ginseng Research

Article Title: Stereospecific anticancer effects of ginsenoside Rg3 epimers isolated from heat-processed American ginseng on human gastric cancer cell

doi: 10.1016/j.jgr.2013.11.007

Figure Lengend Snippet: Changes in effects of ginsenosides Rb1 and Re upon heat-processing on AGS cell proliferation. (A) Cells were treated with ginsenoside Rb1 or heat-processed ginsenoside Rb1 at different concentrations (12.5 μg/mL, 25 μg/mL, 50 μg/mL, and 100 μg/mL) for 24 h. (B) Cells were treated with ginsenoside Re or heat-processed ginsenoside Re at different concentrations (12.5 μg/mL, 25 μg/mL, 50 μg/mL, and 100 μg/mL) for 24 h. Relative cell proliferation was measured by the CCK-8 assay. Each value represents the mean ± standard deviation of three independent experiments. (C) HPLC chromatogram of ginsenoside Rb1 prior to heat-processing. (D) HPLC chromatogram of ginsenoside Rb1 after heat-processing. * p < 0.05 compared with vehicle control. CCK-8, Cell Counting Kit-8; HPLC, high performance liquid chromatography.

Article Snippet: The human gastric cancer AGS cell line was purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: CCK-8 Assay, Standard Deviation, Control, Cell Counting, High Performance Liquid Chromatography

Journal: Journal of Ginseng Research

Article Title: Stereospecific anticancer effects of ginsenoside Rg3 epimers isolated from heat-processed American ginseng on human gastric cancer cell

doi: 10.1016/j.jgr.2013.11.007

Figure Lengend Snippet: Anticancer effects of ginsenosides on AGS cell proliferation. (A) Cells were treated with ginsenoside 20( S,R )-Rg3 at different concentrations (12.5 μg/mL, 25 μg/mL, 50 μg/mL, and 100 μg/mL) for 24 h. (B) Cells were treated with ginsenoside Rg5/Rk1 at different concentrations (12.5 μg/mL, 25 μg/mL, 50 μg/mL, and 100 μg/mL) for 24 h. (C) Cells were treated with ginsenoside 20( S )-Rg3 at different concentrations (6.25 μg/mL, 12.5 μg/mL, 25 μg/mL, 50 μg/mL, and 100 μg/mL) for 24 h. (D) Cells were treated with ginsenoside 20( R )-Rg3 at different concentrations (6.25 μg/mL, 12.5 μg/mL, 25 μg/mL, 50 μg/mL, and 100 μg/mL) for 24 h. Relative cell proliferation was measured by the CCK-8 assay. Each value represents the mean ± standard deviation of three independent experiments. * p < 0.05 compared with the vehicle control. CCK-8, Cell Counting Kit-8.

Article Snippet: The human gastric cancer AGS cell line was purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: CCK-8 Assay, Standard Deviation, Control, Cell Counting

Journal: Otolaryngologic clinics of North America

Article Title: Oral Cavity and Oropharyngeal Squamous Cell Carcinoma Genomics

doi: 10.1016/j.otc.2013.04.001

Figure Lengend Snippet: Selected studies using standard or array comparative genomic hybridization in HNSCC

Article Snippet: 87 Liu et al., 2011 Oligo GEArray Human Cancer Microarray (SuperArray) 440 3 oral SCC, 3 leukoplakia, 3 normal mucosa Identified a panel of 9 genes that differentiated between normal mucosa, oral leukoplakia, and invasive carcinoma.

Techniques: Hybridization, Amplification, Migration, Gene Expression, Over Expression, Microarray

Journal: Otolaryngologic clinics of North America

Article Title: Oral Cavity and Oropharyngeal Squamous Cell Carcinoma Genomics

doi: 10.1016/j.otc.2013.04.001

Figure Lengend Snippet: Selected studies using gene expression arrays in HNSCC

Article Snippet: 87 Liu et al., 2011 Oligo GEArray Human Cancer Microarray (SuperArray) 440 3 oral SCC, 3 leukoplakia, 3 normal mucosa Identified a panel of 9 genes that differentiated between normal mucosa, oral leukoplakia, and invasive carcinoma.

Techniques: Gene Expression, Biomarker Discovery, Expressing, Microarray, Quantitative Proteomics